10th Dental Facial Cosmetic International Conference

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Dye Extraction Method to Evaluate Apical Microleakage of GIC, MTA and Biodentine as a Retrograde Filling Material


The purpose of root-end filling material is to establishan impermeable seal of all the apical avenues of the root canal system andprevent the percolation of bacteria or their products between the root canalsystems and periradicular tissues. Apical microleakage may be the major factorfor the failure of the surgical endodontic treatment. The present studycompares the apical microleakage of three different root-end filling materials.

Materials and methods

50 extracted single rooted maxillary and mandibularpremolars were taken and root canal treatment was completed. Apical 3 mm of allthe teeth were resected with diamond disk. The prepared specimens were dividedrandomly into negative controls (n = 10) and positive controls (n = 10); thenegative controls were completely covered with two layers of nail varnish, andthe positive controls were without root-end preparation and without root-endfilling. In the remaining 30 teeth, retrograde cavity of 3 mm depth wasprepared with round diamond bur and the samples were then randomly divided intothree groups of 10 teeth each, to be filled as the following: Group I : GIC,Group II: MTA, Group III: Biodentine. Apical leakage evaluation was done by dyeextraction methods. Data were statistically analyzed.


The mean±S.D OD were 0.130±0.029 and 0.948±0.287 for negative andpositive control groups respectively. The mean±S.D OD were 0.557±0.095 for GIC,0.254±0.055 for MTA and 0.153±0.038 for Biodentine. The statisticallysignificant difference was observed in between GIC & MTA (p=0.0001) and GIC& Biodentine (p=0.0001) but statistically non-significant difference wasobserved between MTA and Biodentine (p=0.496) respectively.


It can be concluded that Biodentine and MTA showed lessmicroleakage as compared to GIC. There is no significant difference betweenmean microleakage of MTA and Biodentine. However, the mean OD of the biodentinewas least of all evaluated materials.

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