10th Dental Facial Cosmetic International Conference

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Comparison between Surgical Suture Materials in Bacterial Adhesion

Introduction

In dental practice, various suturing materials are widely used to close the wounds and improve healing. Due to the likelihood of adherence of bacteria to the suturing material, microbial colonization usually result leading wound infections. It has been shown that microbial adherence to suture material is highly variable, dependent on the specific microbial species, suture structure, and chemical composition of the suture.

Objective

To compare the antimicrobial effect of three different sutures that are commonly used against 2 bacteria and one fungus (Candida albicans, Escherichia coli and Streptococcus pyogenes)

Methodology

Three commercially available suture materials (silk, polyglycolic acid and antibacterial polyglactin suture),the sutures were aseptically cut into 1-cm lengths and store at room temperature until use. All strains were incubated overnight on selective culture media at 35° C. selective culture media were prepared by inoculating fresh colonies to 20-mL of tryptic soy broth (TSB) and incubating the tubes for 18 hours at 35° C.Standardized inocula, were prepared in 0.85% PBS with 0.50% dextrose. Then individual suture segments were placed in 5-mL tubes and exposed to the diluted inoculum. After exposure to test strains, suture segments were washed (3X), sonicated, serially diluted, plated to culture media, and incubated for 48 hours at 35° C. Microbial recovery was expressed a log10 cfu/cm suture segment. ANOVA was used to compare the CFUs of the strains according to different suture materials.

Results

Significantly less CFU of C. albicans were observed for polyglycolic acid suture than other two suture material. polyglycolic acid suture showed lessE.coli and S. pyogenes adhesion expressed by CFU than other two suture materials.

Conclusion

Polyglycolic acid suture doesn’t favor bacterial adhesion compared to silk and polyglactin suture material.

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